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A comparison of co-culture with oviductal epithelial cells and growth on porcine zygote medium (PZM) on porcine embryos development
- Source :
- Science Technology and Engineering Journal (STEJ), Vol 8, Iss 2, Pp 67-75 (2022)
- Publication Year :
- 2022
- Publisher :
- Mahasarakham University, 2022.
-
Abstract
- This study examined the effect of a chemically defined media system and co-culture system on the in vitro production of porcine embryos. Cumulus-oocyte complexes (COCs) from porcine ovaries were cultured for 22 h in TCM-199 with Earle’s salts, L-glutamine, and NaHCO3 supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/ml), streptomycin (100 μg/ml), 10 IU/ml of human chorionic gonadotropin, 25 μg/ml of follicle-stimulating hormone, 0.2 mM sodium pyruvate. But without the hormone for 22-44 h in vitro maturation (IVM) media. Matured oocytes were fertilized in basal IVF-media (modified Tris-buffered medium; mTBM). The porcine zygotes were randomly cultured in different culture conditions as follows: (1)a chemically defined media with porcine zygote medium (PZM), and (2) co-culture with the oviductal epithelial cells for embryo development. The results showed that the percentage of >4-cells, >16-cells developments and blastocysts in the PZM group (71.67%, 45.97%, and 24.33%, respectively) were higher than in the co-culture group (53.05%, 22.47%, and 8.85%, respectively) (p0.05). These results indicated that in vitro production of porcine embryos using a chemically defined medium significantly increased the percentage of >4-cells, >16-cells development, and the blastocyst stage.
Details
- Language :
- English
- ISSN :
- 26976110 and 26976072
- Volume :
- 8
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Science Technology and Engineering Journal (STEJ)
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.b010402f8c3842b0b73ff755760941d6
- Document Type :
- article