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Development and Characterization of Double-Antibody Sandwich ELISA for Detection of Zika Virus Infection

Authors :
Liding Zhang
Xuewei Du
Congjie Chen
Zhixin Chen
Li Zhang
Qinqin Han
Xueshan Xia
Yuzhu Song
Jinyang Zhang
Source :
Viruses, Vol 10, Iss 11, p 634 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Zika virus (ZIKV) is an emerging mosquito-transmitted flavivirus that can cause severe disease, including congenital birth defect and Guillain−Barré syndrome during pregnancy. Although, several molecular diagnostic methods have been developed to detect the ZIKV, these methods pose challenges as they cannot detect early viral infection. Furthermore, these methods require the extraction of RNA, which is easy to contaminate. Nonstructural protein 1 (NS1) is an important biomarker for early diagnosis of the virus, and the detection methods associated with the NS1 protein have recently been reported. The aim of this study was to develop a rapid and sensitive detection method for the detection of the ZIKV based on the NS1 protein. The sensitivity of this method is 120 ng mL−1 and it detected the ZIKV in the supernatant and lysates of Vero and BHK cells, as well as the sera of tree shrews infected with the ZIKV. Without the isolation of the virus and the extraction of the RNA, our method can be used as a primary screening test as opposed to other diagnosis methods that detect the ZIKV.

Details

Language :
English
ISSN :
19994915
Volume :
10
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.9f4d4c42fe884e2d98d00be43783d133
Document Type :
article
Full Text :
https://doi.org/10.3390/v10110634