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Pitfalls in Monitoring Mitochondrial Temperature Using Charged Thermosensitive Fluorophores

Authors :
Dominique Chrétien
Paule Bénit
Christine Leroy
Riyad El-Khoury
Sunyou Park
Jung Yeol Lee
Young-Tae Chang
Guy Lenaers
Pierre Rustin
Malgorzata Rak
Source :
Chemosensors, Vol 8, Iss 4, p 124 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Mitochondria are the source of internal heat which influences all cellular processes. Hence, monitoring mitochondrial temperature provides a unique insight into cell physiology. Using a thermosensitive fluorescent probe MitoThermo Yellow (MTY), we have shown recently that mitochondria within human cells are maintained at close to 50 °C when active, increasing their temperature locally by about 10 °C. Initially reported in the HEK293 cell line, we confirmed this finding in the HeLa cell line. Delving deeper, using MTY and MTX (MitoThermo X), a modified version of MTY, we unraveled some caveats related to the nature of these charged fluorophores. While enabling the assessment of mitochondrial temperature in HEK and HeLa cell lines, the reactivity of MTY to membrane potential variations in human primary skin fibroblasts precluded local temperature monitoring in these cells. Chemical modification of MTY into MTX did not result in a temperature probe unresponsive to membrane potential variations that could be universally used in any cell type to determine mitochondrial temperature. Thus, the cell-type dependence of MTY in measuring mitochondrial temperature, which is likely due to the variable binding of this dye to specific internal mitochondrial components, should imply cautiousness while using these nanothermometers for mitochondrial temperature analysis.

Details

Language :
English
ISSN :
22279040
Volume :
8
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Chemosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.9f2c598fc497b9e3fb1c4be9c9f46
Document Type :
article
Full Text :
https://doi.org/10.3390/chemosensors8040124