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Fgf8a mutation affects craniofacial development and skeletal gene expression in zebrafish larvae
- Source :
- Biology Open, Vol 8, Iss 9 (2019)
- Publication Year :
- 2019
- Publisher :
- The Company of Biologists, 2019.
-
Abstract
- Craniofacial development is tightly regulated and therefore highly vulnerable to disturbance by genetic and environmental factors. Fibroblast growth factors (FGFs) direct migration, proliferation and survival of cranial neural crest cells (CNCCs) forming the human face. In this study, we analyzed bone and cartilage formation in the head of five dpf fgf8ati282 zebrafish larvae and assessed gene expression levels for 11 genes involved in these processes. In addition, in situ hybridization was performed on 8 and 24 hours post fertilization (hpf) larvae (fgf8a, dlx2a, runx2a, col2a1a). A significant size reduction of eight out of nine craniofacial cartilage structures was found in homozygous mutant (6–36%, P
Details
- Language :
- English
- ISSN :
- 20466390
- Volume :
- 8
- Issue :
- 9
- Database :
- Directory of Open Access Journals
- Journal :
- Biology Open
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9eeabb194aa04c65b2f165c8fe5301f7
- Document Type :
- article
- Full Text :
- https://doi.org/10.1242/bio.039834