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A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice

Authors :
Hongwei Chen
Qi Wang
Mingqian Fan
Xijuan Zhang
Pulin Feng
Lin Zhu
Jiayi Wu
Xiaoyi Cheng
Jiayu Wang
Source :
International Journal of Molecular Sciences, Vol 24, Iss 6, p 5796 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Chloroplasts are essential sites for plant photosynthesis, and the biogenesis of the photosynthetic complexes involves the interaction of nuclear genes and chloroplast genes. In this study, we identified a rice pale green leaf mutant, crs2. The crs2 mutant showed different degrees of low chlorophyll phenotypes at different growth stages, especially at the seedling stage. Fine mapping and DNA sequencing of crs2 revealed a single nucleotide substitution (G4120A) in the eighth exons of CRS2, causing a G-to-R mutation of the 229th amino acid of CRS2 (G229R). The results of complementation experiments confirmed that this single-base mutation in crs2 is responsible for the phenotype of the crs2 mutant. CRS2 encodes a chloroplast RNA splicing 2 protein localized in the chloroplast. Western blot results revealed an abnormality in the abundance of the photosynthesis-related protein in crs2. However, the mutation of CRS2 leads to the enhancement of antioxidant enzyme activity, which could reduce ROS levels. Meanwhile, with the release of Rubisco activity, the photosynthetic performance of crs2 was improved. In summary, the G229R mutation in CRS2 causes chloroplast protein abnormalities and affects photosystem performance in rice; the above findings facilitate the elucidation of the physiological mechanism of chloroplast proteins affecting photosynthesis.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
24
Issue :
6
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.9ee7a3e3b2f44b8a81369afa393ee50e
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms24065796