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Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease

Authors :
Joshua Millar
Luke Gibbons
Catia Costa
Ella Schneider
Johanna von Gerichten
Melanie J. Bailey
Susan Campbell
Catherine Duckett
Sarah Doyle
Laura M. Cole
Source :
Analytica, Vol 4, Iss 3, Pp 264-279 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (Rho−/−) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and Rho−/− tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).

Details

Language :
English
ISSN :
26734532
Volume :
4
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Analytica
Publication Type :
Academic Journal
Accession number :
edsdoj.9d9cdaae364613b307ba81de363f2a
Document Type :
article
Full Text :
https://doi.org/10.3390/analytica4030021