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Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease
- Source :
- Analytica, Vol 4, Iss 3, Pp 264-279 (2023)
- Publication Year :
- 2023
- Publisher :
- MDPI AG, 2023.
-
Abstract
- The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (Rho−/−) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and Rho−/− tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).
- Subjects :
- multimodal
laser ablation
ICP-MS
PIXE
IBA
ocular tissue
Analytical chemistry
QD71-142
Subjects
Details
- Language :
- English
- ISSN :
- 26734532
- Volume :
- 4
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Analytica
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9d9cdaae364613b307ba81de363f2a
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/analytica4030021