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Establishment of primary keratinocyte culture from horse tissue biopsates

Establishment of primary keratinocyte culture from horse tissue biopsates

Authors :
Jernej OGOREVC
Tjaša LAPANJA
Klavdija POKLUKAR
Natalija TOMINŠEK
Peter DOVČ
Source :
Acta Agriculturae Slovenica, Vol 106, Iss 2 (2015)
Publication Year :
2015
Publisher :
University of Ljubljana Press (Založba Univerze v Ljubljani), 2015.

Abstract

Primary cell lines established from skin tissue can be used in immunological, proteomic and genomic studies as in vitro skin models. The goal of our study was to establish a primary keratinocyte cell culture from tissue biopsates of two horses. The primary keratinocyte cell culture was obtained by mechanical and enzymatic dissociation and with explant culture method. The result was a heterogeneous primary culture comprised of keratinocytes and fibroblasts. To distinguish epithelial and mesenchymal cells immunofluorescent characterisation was performed, using antibodies against cytokeratin 14 and vimentin. We successfully at attained a primary cell line of keratinocytes, which could potentially be used to study equine skin diseases, as an animal model for human diseases, and for cosmetic and therapeutic product testing.

Details

Language :
English, Slovenian
ISSN :
18541941
Volume :
106
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Acta Agriculturae Slovenica
Publication Type :
Academic Journal
Accession number :
edsdoj.9cfd7ebcf2314c56ba8157145f4ed305
Document Type :
article
Full Text :
https://doi.org/10.14720/aas.2015.106.2.3