Back to Search
Start Over
Establishment of primary keratinocyte culture from horse tissue biopsates
Establishment of primary keratinocyte culture from horse tissue biopsates
- Source :
- Acta Agriculturae Slovenica, Vol 106, Iss 2 (2015)
- Publication Year :
- 2015
- Publisher :
- University of Ljubljana Press (Založba Univerze v Ljubljani), 2015.
-
Abstract
- Primary cell lines established from skin tissue can be used in immunological, proteomic and genomic studies as in vitro skin models. The goal of our study was to establish a primary keratinocyte cell culture from tissue biopsates of two horses. The primary keratinocyte cell culture was obtained by mechanical and enzymatic dissociation and with explant culture method. The result was a heterogeneous primary culture comprised of keratinocytes and fibroblasts. To distinguish epithelial and mesenchymal cells immunofluorescent characterisation was performed, using antibodies against cytokeratin 14 and vimentin. We successfully at attained a primary cell line of keratinocytes, which could potentially be used to study equine skin diseases, as an animal model for human diseases, and for cosmetic and therapeutic product testing.
- Subjects :
- molecular biology
primary cell culture
skin
horses
keratinocytes
Agriculture
Subjects
Details
- Language :
- English, Slovenian
- ISSN :
- 18541941
- Volume :
- 106
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Acta Agriculturae Slovenica
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9cfd7ebcf2314c56ba8157145f4ed305
- Document Type :
- article
- Full Text :
- https://doi.org/10.14720/aas.2015.106.2.3