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Development of a Highly Sensitive Technique for Capturing Renal Cell Cancer Circulating Tumor Cells

Authors :
Michio Naoe
Chiho Kusaka
Mika Ohta
Yuki Hasebe
Tsutomu Unoki
Hideaki Shimoyama
Takehiko Nakasato
Kazuhiko Oshinomi
Jun Morita
Kohzo Fuji
Yoshio Ogawa
Mana Tsukada
Masataka Sunagawa
Hikaru Ishii
Source :
Diagnostics, Vol 9, Iss 3, p 96 (2019)
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

purpose: Liquid biopsy is becoming increasingly important as a guide for selecting new drugs and determining their efficacy. In urological cancer, serum markers for renal cell and urothelial cancers has made the development of liquid biopsy for these cancers strongly desirable. Liquid biopsy is less invasive than conventional tissue biopsy is, enabling frequent biopsies and, therefore, is considered effective for monitoring the treatment course. Circulating tumor cells (CTCs) are a representative liquid biopsy specimen. In the present study, we focused on developing our novel technology for capturing renal cell cancer (RCC)-CTCs using an anti-G250 antibody combined with new devices. Basic experiments of our technology showed that it was possible to detect RCC-CTC with a fairly high accuracy of about 95%. Also, RCC-CTC was identified in the peripheral blood of actual RCC patients. Additionally, during the treatment course of the RCC patient, change in the number of RCC-CTC was confirmed in one case. We believe that the technology we developed will be useful for determining the treatment efficacy and drug selection for the treatment of renal cell cancer (RCC). In order to solve issues such as thresholds setting of this technology, large-scale clinical trials are expected.

Details

Language :
English
ISSN :
20754418
Volume :
9
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Diagnostics
Publication Type :
Academic Journal
Accession number :
edsdoj.9be5152dc3dc450ca8f78b8ed3d504e3
Document Type :
article
Full Text :
https://doi.org/10.3390/diagnostics9030096