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Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase [version 1; peer review: 2 approved, 1 approved with reservations]

Authors :
Ulrich Dobramysl
Tom Beneke
Jeremy Charles Mottram
Carolina Moura Costa Catta-Preta
Richard Wheeler
Eva Gluenz
Source :
Wellcome Open Research, Vol 7 (2022)
Publication Year :
2022
Publisher :
Wellcome, 2022.

Abstract

We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.

Details

Language :
English
ISSN :
2398502X
Volume :
7
Database :
Directory of Open Access Journals
Journal :
Wellcome Open Research
Publication Type :
Academic Journal
Accession number :
edsdoj.9baa413bf1a5409da097c9d557de78f5
Document Type :
article
Full Text :
https://doi.org/10.12688/wellcomeopenres.18575.1