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Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase [version 1; peer review: 2 approved, 1 approved with reservations]
- Source :
- Wellcome Open Research, Vol 7 (2022)
- Publication Year :
- 2022
- Publisher :
- Wellcome, 2022.
-
Abstract
- We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
- Subjects :
- Leishmania mexicana
Genome
Pilon polish
eng
Medicine
Science
Subjects
Details
- Language :
- English
- ISSN :
- 2398502X
- Volume :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- Wellcome Open Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9baa413bf1a5409da097c9d557de78f5
- Document Type :
- article
- Full Text :
- https://doi.org/10.12688/wellcomeopenres.18575.1