High-Fat and High-Sucrose Diet Leads to Skeletal Muscle Loss and Bladder Dysfunction in Rat

Naoki Wada, Noriyuki Abe, Kotona Miyauchi, Shogo Makino, Hidehiro Kakizaki Department of Renal and Urologic Surgery, Asahikawa Medical University, Asahikawa, JapanCorrespondence: Naoki Wada, Department of Renal and Urologic Surgery, Asahikawa Medical University, Asahikawa, Japan, Tel +81-166-65-2533, Email nwada@asahikawa-med.ac.jpPurpose: In this study, we investigated skeletal muscle loss and bladder dysfunction caused by high-fat/high-sucrose (HFS) diet.Methods: Twelve-week-old Sprague–Dawley (SD) female rats were fed on normal (Group N) or HFS (Group HFS) diet for 12 weeks. We conducted urodynamic investigation and pharmacologic in vitro. In addition, we measured gastrocnemius and tibialis muscle weight and protein concentration. The hypoxia-inducible factor (HIF)-1α and 8-hydroxy-2’-deoxyguanosine (8-OHdG) in the bladder were assayed.Results: The urodynamic investigations revealed the significantly shorter intercontraction intervals and lower maximal voiding pressure in Group HFS than in Group N. Furthermore, the absolute and relative weights of the gastrocnemius muscle were found to be significantly lower in Group HFS than in Group N. The protein concentration of the gastrocnemius muscle was also significantly lower in Group HFS than in Group N. The absolute and relative weights of the bladder were also significantly lower in Group HFS than in Group N. The contractile responses of the bladder strips to electrical field stimulation and carbachol were significantly lower in Group HFS than in Group N. The HIF1α and 8OHdG in the bladder muscle were significantly higher in Group HFS than in Group N. The HFS diet reduced bladder capacity and contractility along with the loss of the gastrocnemius muscle.Conclusion: HFS diet promotes bladder dysfunction similar to detrusor hyperreflexia with impaired contractility.Keywords: high-fat, high-sucrose, bladder dysfunction, rat, skeletal muscle