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Bio-Sulfur Pre-Treatment Suppresses Anthracnose on Cucumber Leaves Inoculated with Colletotrichum orbiculare
- Source :
- Mycobiology, Vol 47, Iss 3, Pp 308-318 (2019)
- Publication Year :
- 2019
- Publisher :
- Taylor & Francis Group, 2019.
-
Abstract
- Bio-sulfur can be produced in the process of desulfurization from a landfill and collected by some microorganism such as Thiobacillus sp. as a sulfur element. In order to investigate practical use of bio-sulfur as an agent for controlling plant disease, in vitro antifungal activity of bio-sulfur was tested against Colletotrichum orbiculare known to cause cucumber anthracnose. Efficacy of bio-sulfur for suppressing anthracnose disease was also evaluated in vivo using cucumber leaves. Mycelial growth of C. orbiculare on medium containing bio-sulfur was inhibited. Disease severity of cucumber leaves pre-treated with bio-sulfur was significantly decreased compared to that of untreated ones. To illustrate how bio-sulfur could suppress anthracnose disease, structures of cucumber leaves infected with C. orbiculare were observed under a fluorescent microscope and a scanning electron microscope (SEM). Cucumber leaves pre-treated with bio-sulfur showed a low rate of appressorium formation whereas untreated ones showed abundant appressoria. Shrunk fungal hyphae were mostly observed on bio-sulfur-pretreated leaves by SEM. Similar results were observed on leaves pre-treated with a commercial fungicide Benomyl®. These results suggest that inhibition of appressorium formation of C. orbiculare by bio-sulfur may contribute to its suppression of cucumber anthracnose.
- Subjects :
- Environment-friendly
fluorescence microscope
infection behavior
Botany
QK1-989
Subjects
Details
- Language :
- English
- ISSN :
- 12298093 and 20929323
- Volume :
- 47
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Mycobiology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.999fd6bf4ea4157ac223ec5c87ec131
- Document Type :
- article
- Full Text :
- https://doi.org/10.1080/12298093.2019.1628522