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A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem

Authors :
Bihai Shi
Amelia Felipo-Benavent
Guillaume Cerutti
Carlos Galvan-Ampudia
Lucas Jilli
Geraldine Brunoud
Jérome Mutterer
Elody Vallet
Lali Sakvarelidze-Achard
Jean-Michel Davière
Alejandro Navarro-Galiano
Ankit Walia
Shani Lazary
Jonathan Legrand
Roy Weinstain
Alexander M. Jones
Salomé Prat
Patrick Achard
Teva Vernoux
Source :
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)
Publication Year :
2024
Publisher :
Nature Portfolio, 2024.

Abstract

Abstract Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
15
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.9986d747207c44faba63b699c97c4c89
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-024-48116-4