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Towards a portable setup for the on-site SERS detection of miRNAs
- Source :
- Journal of the European Optical Society-Rapid Publications, Vol 20, Iss 1, p 28 (2024)
- Publication Year :
- 2024
- Publisher :
- EDP Sciences, 2024.
-
Abstract
- The actual implementation of on-site analysis of biomarkers, such as miRNAs, depends on the availability of portable and easy-to-handle detection systems that can be used as point-of-care in real life applications. In this work, an automatized microfluidic platform combined with a portable Raman spectrometer is reported and tested for miR-214 detection by surface enhanced Raman scattering (SERS). A multi-chamber SERS-active chip, functionalized according to a previously optimized two-step hybridization assay, was automatically incubated with the analyte solution. Preliminary tests allowed to adjust the portable Raman spectrometer acquisition conditions and to compare the obtained results with those of a bench Raman microscope. Finally, miR-214 at different concentrations was analyzed through an automatic procedure, achieving a limit of detection (LOD) in the picomolar range.
Details
- Language :
- English
- ISSN :
- 19902573
- Volume :
- 20
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of the European Optical Society-Rapid Publications
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9922c643f7e04876b9a690fc33c46fa6
- Document Type :
- article
- Full Text :
- https://doi.org/10.1051/jeos/2024028