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Towards a portable setup for the on-site SERS detection of miRNAs

Authors :
Montesi Daniel
Bertone Sofia
Rivolo Paola
Geobaldo Francesco
Giorgis Fabrizio
Novara Chiara
Chiadò Alessandro
Source :
Journal of the European Optical Society-Rapid Publications, Vol 20, Iss 1, p 28 (2024)
Publication Year :
2024
Publisher :
EDP Sciences, 2024.

Abstract

The actual implementation of on-site analysis of biomarkers, such as miRNAs, depends on the availability of portable and easy-to-handle detection systems that can be used as point-of-care in real life applications. In this work, an automatized microfluidic platform combined with a portable Raman spectrometer is reported and tested for miR-214 detection by surface enhanced Raman scattering (SERS). A multi-chamber SERS-active chip, functionalized according to a previously optimized two-step hybridization assay, was automatically incubated with the analyte solution. Preliminary tests allowed to adjust the portable Raman spectrometer acquisition conditions and to compare the obtained results with those of a bench Raman microscope. Finally, miR-214 at different concentrations was analyzed through an automatic procedure, achieving a limit of detection (LOD) in the picomolar range.

Details

Language :
English
ISSN :
19902573
Volume :
20
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Journal of the European Optical Society-Rapid Publications
Publication Type :
Academic Journal
Accession number :
edsdoj.9922c643f7e04876b9a690fc33c46fa6
Document Type :
article
Full Text :
https://doi.org/10.1051/jeos/2024028