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Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology

Authors :
Angélica Hernández-Arriaga
Anja Baumann
Otto W. Witte
Christiane Frahm
Ina Bergheim
Amélia Camarinha-Silva
Source :
Microorganisms, Vol 7, Iss 9, p 283 (2019)
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

The mouth is an important niche for bacterial colonization. Previous research used mouth microbiota to predict diseases like colon cancer and inflammatory bowel disease (IBD). It is still unclear how the sampling methodology influences microbial characterization. Our aim was to determine if the sampling methods, e.g., cotton swab or tissue biopsy, and the age influence the oral microbial composition of mice. Microbial DNA was extracted using a commercial kit and characterized targeting the 16s rRNA gene from mouth swabs and tissue biopsies from 2 and 15 months old C57BL/6 male mice kept in the same SPF facility. Our results show statistical different microbial community of the different ages, type of sampling, and the two fixed factors age x type of sample (p-value < 0.05). At the genus level, we identified that the genera Actinobacillus, Neisseria, Staphylococcus, and Streptococcus either increase or decrease in abundance depending on sampling and age. Additionally, the abundance of Streptococcus danieliae, Moraxella osloensis, and some unclassified Streptococcus was affected by the sampling method. While swab and tissue biopsies both identified the common colonizers of oral microbiota, cotton swabbing is a low-cost and practical method, validating the use of the swab as the preferred oral sampling approach.

Details

Language :
English
ISSN :
20762607
Volume :
7
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.98c875aa8c1d4c60ba3172994a8159ae
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms7090283