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SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro

SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro

Authors :
Marcio da Costa Cipitelli
Elizabeth Valentin
Nadia Vaez Gonçalves da Cruz
Tatiana LS Nogueira
Elaine Cristina Amaro de Melo
Rebeca Araujo da Silva
Marcelo M Serra
André L Meriano
Alberto ML Colares
Marcos Dornelas-Ribeiro
Caleb GM Santos
Source :
Memorias do Instituto Oswaldo Cruz, Vol 115 (2020)
Publication Year :
2020
Publisher :
Fundação Oswaldo Cruz (FIOCRUZ), 2020.

Abstract

Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.

Details

Language :
English
ISSN :
16788060 and 00740276
Volume :
115
Database :
Directory of Open Access Journals
Journal :
Memorias do Instituto Oswaldo Cruz
Publication Type :
Academic Journal
Accession number :
edsdoj.97fbb7be7eed4e03a9cec9f091031546
Document Type :
article
Full Text :
https://doi.org/10.1590/0074-02760200200