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Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing
- Source :
- Viruses, Vol 16, Iss 8, p 1216 (2024)
- Publication Year :
- 2024
- Publisher :
- MDPI AG, 2024.
-
Abstract
- Transduction of producer cells during lentiviral vector (LVV) production causes the loss of 70–90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the LDL receptor located on the producer cell membrane, allowing lentiviral vector transduction. Avoiding retro-transduction in LVV manufacturing is crucial to improve net production and, therefore, the efficiency of the production process. Here, we describe a method for quantifying the transduction of producer cells and three different strategies that, focused on the interaction between VSV-G and the LDLR, aim to reduce retro-transduction.
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 16
- Issue :
- 8
- Database :
- Directory of Open Access Journals
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.975fd8c332864cfb987c2323f6cf215f
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/v16081216