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Surveillance, isolation and complete genome sequence of bovine parainfluenza virus type 3 in Egyptian cattle
- Source :
- International Journal of Veterinary Science and Medicine, Vol 5, Iss 1, Pp 8-13 (2017)
- Publication Year :
- 2017
- Publisher :
- Taylor & Francis Group, 2017.
-
Abstract
- Parainfluenza virus type 3 (PIV-3) can infect a wide variety of mammals including humans, domestic animals, and wild animals. In the present study, bovine parainfluenza virus type 3 (BPIV-3) was isolated from nasal swabs of Egyptian cattle presenting with clinical signs of mild pneumonia. The virus was isolated in Madin-Darby bovine kidney (MDBK) cells and confirmed by reverse transcription-polymerase chain reaction (RT-PCR). The complete genome of Egyptian BPIV-3 strain was sequenced by using next generation (Illumina) sequencing. The new isolate classified with genotype A of BPIV-3 and was closely related to the Chinese NM09 strain (JQ063064). Subsequently in 2015–16, a molecular surveillance study was undertaken by collecting and testing samples from cattle and buffaloes with respiratory tract infections. The survey revealed a higher rate of BPIV-3 infection in cattle than in buffaloes. The infection was inversely proportional to the age of the animals and to warm weather. This report should form a basis for further molecular studies on animal viruses in Egypt.
- Subjects :
- BPIV-3
Egypt
Cattle
Phylogenetic analysis
Parainfluenza
Veterinary medicine
SF600-1100
Subjects
Details
- Language :
- English
- ISSN :
- 23144599
- Volume :
- 5
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- International Journal of Veterinary Science and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.96ea96c3cd4168850f5413da995aab
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.ijvsm.2017.02.004