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In Vitro Digestion of Vacuum-Impregnated Yam Bean Snacks: Pediococcus acidilactici Viability and Mango Seed Polyphenol Bioaccessibility

Authors :
Alba Cecilia Durán-Castañeda
Adela Yolanda Bueno-Durán
Manuel Iván Girón-Pérez
Juan Arturo Ragazzo-Sánchez
Jorge Alberto Sánchez-Burgos
Sonia Guadalupe Sáyago-Ayerdi
Victor Manuel Zamora-Gasga
Source :
Microorganisms, Vol 12, Iss 10, p 1993 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

This study investigates the in vitro digestion of vacuum-impregnated yam bean snacks enriched with Pediococcus acidilactici and mango seed polyphenols, focusing on bacterial survival and polyphenol bioaccessibility. The snacks were prepared by vacuum impregnation (VI) with solutions containing either mango seed extract, P. acidilactici, or a combination of both, followed by dehydration. The antimicrobial activity of the treatments was assessed against pathogens, revealing limited effectiveness, likely due to insufficient concentrations of mango seed extract and the intrinsic resistance of the bacteria. VI of mango seed extract improved the total soluble phenols (TSP) content up to 400% and maintained the initial probiotic concentration (106 cell/mL). In vitro digestion was performed to simulate gastrointestinal conditions, measuring the stability of TSP and the survival of P. acidilactici. The results indicated that the viability of P. acidilactici fluctuated throughout the digestion process (106 to 104 log UFC/g), the polyphenols showed varying degrees of bioaccessibility (11 to 30%), and the TSP content in the intestinal fraction ranged from 1.95 to 6.54 mg GAE/g. The study highlights the potential of VI for incorporating functional components into plant-based snacks, though further optimization is necessary to enhance the stability of P. acidilactici and the effectiveness of the bioactive ingredients.

Details

Language :
English
ISSN :
20762607
Volume :
12
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.96e4204ac3d84c48b6033608c9f79351
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms12101993