Response of Nile tilapia under biofloc system to floating or sinking feed and feeding rates: Water quality, plankton community, growth, intestinal enzymes, serum biochemical and antioxidant status

The current study examined the combined effects of feeding rates and feeding types on the water quality, growth performance, digestive enzymes, blood parameters, and liver antioxidant enzymes of Nile tilapia (Oreochromis niloticus) fingerlings reared under a biofloc system. A 3 × 2 factorial experimental design was used with three feeding rates (2%, 3%, and 4%) and two feeding types (sinking or floating feed), comprising six treatments with three replicates. Fingerlings with an initial body weight of 4.50 ± 0.25 g were stocked in eighteen circular plastic tanks (0.5 m3) at a stocking density of 35 fish per tank fed three times a day at 10:00, 12:00 and 15:00. Starch was added to all treatments as an organic carbon source at a C/N ratio of 10:1. The phytoplankton community was determined to consist of twenty-two species, including individuals from the classes Cyanobacteria, Chlorophyceae, and Bacillariophyta. The most common phytoplankton classes were Chlorophyceae, followed by Cyanobacteria, and fish-fed floating feed at a feeding rate of 2% of total biomass yielded the greatest number of phytoplankton communities. Eight zooplankton species belonging to rotifers and protozoa were identified during this experiment. The highest values of final body weight, weight gain, and specific growth rate were recorded for a fish-fed floating diet with the highest feeding rate (4% of biomass). The highest hepatosomatic index (HIS) was detected in a fish-fed floating diet at a rate of 4% of total biomass. Though fish-fed sinking feed with a 4% biomass feeding rate presented the highest spleen index (SI). The highest significant (P 0.05) on hemoglobin (Hb), hematocrit (Hct), and red blood cells (RBCs). Feeding rate, feed type, and their interactions had no significant impact (P > 0.05) on serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), or albumin, but feeding rates or feed types had an effect (P