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SNHG6 Interacted with miR-325-3p to Regulate Cisplatin Resistance of Gastric Cancer by Targeting GITR
- Source :
- OncoTargets and Therapy, Vol Volume 13, Pp 12181-12193 (2020)
- Publication Year :
- 2020
- Publisher :
- Dove Medical Press, 2020.
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Abstract
- Tuanhe Sun, Kang Li, Kun Zhu, Rong Yan, Chengxue Dang, Dawei Yuan Department of Surgical Oncology, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of ChinaCorrespondence: Dawei YuanDepartment of Surgical Oncology, First Affiliated Hospital of Xi’an Jiaotong University, 277 West Yanta Road, Xi’an, Shaanxi 710061, People’s Republic of ChinaEmail dwyuan@mail.xjtu.edu.cnBackground: Cisplatin resistance results in the failure of platinum-based chemotherapy and relapse of gastric cancer. We aimed to investigate the potential regulating role of SNHG6/miR-325-3p/GITR in reversing cisplatin resistance.Patients and Methods: A total of 137 gastric cancer patients were recruited. qRT-PCR and ELISA were used to test the expression of target genes. CCK-8 and caspase 3/7 kit were used to test the cell viability and apoptosis rate. Dual luciferase reporter gene and RNA-pull down assay were used to investigate the potential interaction between target genes.Results: SNHG6 and GITR were up regulated in gastric cancer; however, miR-325-3p was down-regulated. Besides, SNHG6, miR-325-3p and GITR expression were associated with gastric cancer prognosis. Then, we found that GITR and SNHG6 promoted proliferation and inhibited apoptosis of MKN45 and MKN45 cisplatin resistance cell line; however, miR-325-3p inhibited proliferation and promoted apoptosis of these cell lines. Furthermore, SNHG6 might bind to miR-325-3p to regulate its expression, and miR-325-3p directly interacted with the 3`UTR of GITR.Conclusion: SNHG6 binds to miR-325-3p, which directly interacted with GITR to regulate cisplatin resistance of gastric cancer.Keywords: gastric cancer, cisplatin resistance, GITR, SNHG5, miR-325-3p
Details
- Language :
- English
- ISSN :
- 11786930
- Volume :
- ume 13
- Database :
- Directory of Open Access Journals
- Journal :
- OncoTargets and Therapy
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9690cb7b7f114b5d9554296e5d5bbbcb
- Document Type :
- article