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LncRNA (HOTAIR) /miR-206/TAGLN2 signal axis regulates the invasion of papillarythyroid carcinoma cells
- Source :
- Jichu yixue yu linchuang, Vol 43, Iss 11, Pp 1642-1647 (2023)
- Publication Year :
- 2023
- Publisher :
- Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2023.
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Abstract
- Objective To investigate the molecular mechanism of lncRNA (HOTAIR)/miR-206/TAGLN2 signaling axis in regulating the invasion of papillary thyroid carcinoma cells line TPC-1. Methods The papillary thyroid carcinoma(PTC) and paracancerous tissues were collected from the First Hospital of Lanzhou University, and the expression of lncRNA (HOTAIR), miR-206 and TAGLN2 in PTC tissues as well as paracancerous tissues was detected by RT-qPCR and Western blot; The thyroid papillary cancer cells with miR-206 over-expression and HOTAIR under-expression were constructed to detect the expression of target gene and its effect on the invasion of TPC-1 cells by Transwell assay. Results Compared with the adjacent tissue, the expression of HOTAIR in papilla-rythyroid cancer tissue was increased by 2.48 times. miR-206-5p was decreased down to 0.6,and the expression of TAGLN2 was increased (P<0.05). After over-expression of miR-206, the protein expression of TAGLN2 was decreased in TPC-1 cell, and the invasion of TPC-1 cells was inhibited (P<0.05). Knockdown of HOTAIR increased expression of miR-206 in TPC-1 cells and decreased the expression of TAGLN2. The Transwell assay showed that the invasion of TPC-1 cells was significantly reduced (P<0.05). Conclusions In PTC, reducing the expression of HOTAIR results in over-expression of miR-206 then further inhibits the expression of TAGLN2. The biological outcome is inhibition of invasion of thyroid cancer.
Details
- Language :
- Chinese
- ISSN :
- 10016325
- Volume :
- 43
- Issue :
- 11
- Database :
- Directory of Open Access Journals
- Journal :
- Jichu yixue yu linchuang
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.96573d4fa24d4164a1da768389d35f79
- Document Type :
- article
- Full Text :
- https://doi.org/10.16352/j.issn.1001-6325.2023.11.1642