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Microbial metagenomic shifts in children with acute lymphoblastic leukaemia during induction therapy and predictive biomarkers for infection

Authors :
Huidi Wang
Yajie Zhang
Qianyi Zhou
Lihua Yu
Jingxiang Fu
Danna Lin
Lulu Huang
Xiaorong Lai
Li Wu
Jingxin Zhang
Juan Zi
Xu Liao
Siying Huang
Yugu Xie
Yan He
Lihua Yang
Source :
Annals of Clinical Microbiology and Antimicrobials, Vol 23, Iss 1, Pp 1-10 (2024)
Publication Year :
2024
Publisher :
BMC, 2024.

Abstract

Abstract Background Emerging evidence has indicated a link between the gut microbiota and acute lymphoblastic leukaemia (ALL). However, the acute changes in gut microbiota during chemotherapy and the predictive value of baseline gut microbiota in infectious complication remain largely unknown. Methods Faecal samples (n = 126) from children with ALL (n = 49) undergoing induction chemotherapy were collected at three timepoints, i.e., initiation of chemotherapy (baseline, T0), 7 days (T1) and 33 days (T2) after initiation of chemotherapy. Gut microbiome profile was performed via metagenomic shotgun sequencing. The bioBakery3 pipeline (Kneaddata, Metaphlan 3 and HUMAnN) was performed to assign taxonomy and functional annotations. Gut microbiome at T0 were used to predict infection during chemotherapy. Results The microbial diversities and composition changed significantly during chemotherapy, with Escherichia coli, Klebsiella pneumoniae and Bifidobacterium longum being the most prominent species. The microbial metabolic pathways were also significantly altered during chemotherapy, including the pathway of pyruvate fermentation to acetate and lactate, and assimilatory sulfate reduction pathway. The receiver operating characteristic (ROC) models based on Bifidobacterium longum at T0 could predict infectious complications during the first month of chemotherapy with the area under the curve (AUC) of 0.720. Conclusions Our study provides new insights into the acute changes in microbial and functional characteristics in children with ALL during chemotherapy. The baseline gut microbiota could be potential biomarkers for infections during chemotherapy. Trial registration The study was approved by the Ethics Committee of Zhujiang Hospital, Southern Medical University (2021-KY-171-01) and registered on http://www.chictr.org.cn (ChiCTR2200065406, Registration Date: November 4, 2022).

Details

Language :
English
ISSN :
14760711
Volume :
23
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Annals of Clinical Microbiology and Antimicrobials
Publication Type :
Academic Journal
Accession number :
edsdoj.963d697a63f6470f9a5abb552b31eb94
Document Type :
article
Full Text :
https://doi.org/10.1186/s12941-024-00717-z