Back to Search Start Over

Identification of a specific Vimentin Isoform that induces an antibody response in pancreatic cancer

Authors :
Su-Hyung Hong
David E. Misek
Hong Wang
Eric Puravs
Robert Hinderer
Thomas J. Giordano
Joel K. Greenson
Dean E. Brenner
Diane M. Simeone
Craig D. Logsdon
Samir M. Hanash
Source :
Biomarker Insights, Vol 1, Pp 175-183 (2006)
Publication Year :
2006
Publisher :
SAGE Publishing, 2006.

Abstract

Pancreatic cancer has a poor prognosis, in part due to lack of early detection. The identification of circulating tumor antigens or their related autoantibodies provides a means for early cancer diagnosis. We have used a proteomic approach to identify proteins that commonly induce a humoral response in pancreatic cancer. Proteins from a pancreatic adenocarcinoma cell line (Panc-1) were subjected to two-dimensional PAGE, followed by Western blot analysis in which individual sera were tested for autoantibodies. Sera from 36 newly diagnosed patients with pancreatic cancer, 18 patients with chronic pancreatitis and 15 healthy subjects were analyzed. Autoantibodies were detected against a protein identified by mass spectrometry as vimentin, in sera from 16/36 patients with pancreatic cancer (44.4%). Only one of 18 chronic pancreatitis patients and none of the healthy controls exhibited reactivity against this vimentin isoform. Interestingly, none of several other isoforms of vimentin detectable in 2-D gels exhibited reactivity with patient sera. Vimentin protein expression levels were investigated by comparing the integrated intensity of spots visualized in 2-D PAGE gels of various cancers.Pancreatic tumor tissues showed greater than a 3-fold higher expression of total vimentin protein than did the lung, colon, and ovarian tumors that were analyzed. The specifi c antigenic isoform was found at 5-10 fold higher levels. The detection of autoantibodies to this specific isoform of vimentin may have utility for the early diagnosis of pancreatic cancer.

Subjects

Subjects :
Medicine (General)
R5-920

Details

Language :
English
ISSN :
11772719
Volume :
1
Database :
Directory of Open Access Journals
Journal :
Biomarker Insights
Publication Type :
Academic Journal
Accession number :
edsdoj.9612ef26160d44b1b4cdcaf64f4b8371
Document Type :
article