Back to Search Start Over

Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture

Authors :
Bathini Thissera
Hani A. Alhadrami
Marwa H. A. Hassan
Hossam M. Hassan
Fathy A. Behery
Majed Bawazeer
Mohammed Yaseen
Lassaad Belbahri
Mostafa E. Rateb
Source :
Biomolecules, Vol 10, Iss 2, p 268 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent/cryptic bacterial biosynthetic pathway by the co-culture of Durum wheat plant roots-associated bacterium Pantoea aggolomerans and date palm leaves-derived fungus Penicillium citrinum. The initial co-culture indicated a clear fungal growth inhibition which was confirmed by the promising antifungal activity of the co-culture total extract against Pc. LC-HRMS chemical profiling demonstrated a huge suppression in the production of secondary metabolites (SMs) of axenic cultures of both species with the emergence of new metabolites which were dereplicated as a series of siderophores. Large-scale co-culture fermentation led to the isolation of two new pulicatin derivatives together with six known metabolites which were characterised using HRESIMS and NMR analyses. During the in vitro antimicrobial evaluation of the isolated compounds, pulicatin H (2) exhibited the strongest antifungal activity against Pc, followed by aeruginaldehyde (1) and pulicatin F (4), hence explaining the initial growth suppression of Pc in the co-culture environment.

Details

Language :
English
ISSN :
2218273X and 10020268
Volume :
10
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Biomolecules
Publication Type :
Academic Journal
Accession number :
edsdoj.95dedb9c6f9943e182487df2d0dc3d50
Document Type :
article
Full Text :
https://doi.org/10.3390/biom10020268