Investigation of Toxoplasma gondii and Neospora caninum as cause of ovine abortion in affected flocks of Urmia, northwest of Iran

Toxoplasma gondii and Neospora caninum, two obligatory intracellular protozoan parasites, are im-portant causes of neonatal mortality and abortion in farmed ruminants worldwide. Previously, proto-zoan abortions in small ruminants were attributed to T. gondii, but the role of N. caninum in these abortions was uncertain. The aim of this study was to investigate the role of T. gondii and N. caninum in ovine abortion in Urmia, northwest of Iran using a molecular method. Overall, 130 placenta and brain samples of aborted ovine foetuses were collected. Extracted DNA from placenta and CNS tis-sues of the aborted foetuses were analysed using PCR with primers specific for T. gondii and N. cani¬num. The association of the frequency of T. gondii and N. caninum infection of aborted foetuses with age and breed in flocks was also studied. The results showed that out of the 130 examined ovine foe-tuses, 5.3 and 2.3 % were PCR-positive for T. gondii and N. caninum DNA, respectively. In this study, no significant differences were recorded relating to Toxoplasma and Neospora infection in different age groups in flocks and among sheep breeds included in the present study (P>0.05). The results of this study proved the importance of T. gondii and N. caninum as reasons of abortion in the studied area.