Aqueous Two-Phase Flotation for Extraction of Andrographolide from Andrographis Paniculata

This paper was presented about the extraction of bioactive ingredient that is beneficial towards skin and human health from herbs. Andrographis Paniculata was used for this paper to undergo optimisation of extraction method by modern and novel extraction technique which is aqueous two-phase flotation (ATPF). ATPF extraction is rapid and has simple extraction procedures, resulting in achieving the target yield in least time and energy saving. Under comparison between sonication, soxhlet, aqueous two-phase system (ATPS), and ATPF extraction techniques, ATPF had been chosen as the more effective extraction method for Andrographis Paniculata. The optimised ATPF system was able to yield 5.85 % of Andrographolide with the ATPF’s operating condition set to 38.96 wt% methanol as top phase, 12 wt% di-potassium hydrogen phosphate as bottom phase, 30 min of flotation time, 50 mL/min nitrogen gas flowrate, and 0.75 g of crude load at ambient temperature (25 ± 1 °C).