Detection of Free-Living Amoeba in a tertiary care hospital

This study aims to investigate the presence of Free-Living Amoebae (FLA), particularly Acanthamoeba spp., in diverse water systems and body fluids within a hospital. Water and body fluid samples were collected from various locations. Cultivation of FLA was performed using Non-Nutrient Agar (NNA) culture with Escherichia coli, and microscopic observations after staining. Microscopic observations revealed the presence of Acanthamoeba spp. in pre-dialysis tank water and dental flush water samples. The identification of Acanthamoeba in specific water sources underscores the importance of continuous monitoring and preventive strategies to mitigate potential health risks.