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High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

Authors :
Gregory J. Nierode
Brian C. Perea
Sean K. McFarland
Jorge F. Pascoal
Douglas S. Clark
David V. Schaffer
Jonathan S. Dordick
Source :
Stem Cell Reports, Vol 7, Iss 5, Pp 970-982 (2016)
Publication Year :
2016
Publisher :
Elsevier, 2016.

Abstract

A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.

Details

Language :
English
ISSN :
22136711
Volume :
7
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Stem Cell Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.93896739bb0749189e37a02909ac5839
Document Type :
article
Full Text :
https://doi.org/10.1016/j.stemcr.2016.10.001