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Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA

Authors :
Matthew T Parker
Katarzyna Knop
Vasiliki Zacharaki
Anna V Sherwood
Daniel Tomé
Xuhong Yu
Pascal GP Martin
Jim Beynon
Scott D Michaels
Geoffrey J Barton
Gordon G Simpson
Source :
eLife, Vol 10 (2021)
Publication Year :
2021
Publisher :
eLife Sciences Publications Ltd, 2021.

Abstract

Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.

Details

Language :
English
ISSN :
2050084X
Volume :
10
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.93848093b644c9b91f021cb3118bfcf
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.65537