The effect of aerobic exercise with ozone and stem cell on anandamide concentration in desert rat with osteoarthritis

Background: Aerobic exercise, ozone therapy and cell therapy have each been independently proposed as treatments for osteoarthritis. Therefore, the present study aimed to investigate the effect of aerobic exercise with ozone and cell therapy on serum levels of anandamide concentration in osteoarthritis rats. Materials and Methods: Forty-five male rats were randomly divided into 9 groups, including 1 group: healthy control, 2-patient control, 3-exercise, 4-ozone therapy, 5-cell therapy, 6-exercise+ozone therapy, 7-exercise+cell therapy, 8-ozone+cell therapy and 9- ozone+cell therapy+exercise. Cell groups alone and combination cell therapy received 1×10 6 cells/kg, and ozone groups alone and ozone combination therapies received 20μg/ml of ozone in 3 replications before exercise. The training program consisted of running on a treadmill at a speed of 16 meters per minute for 8 weeks. Forty-eight hours after the last exercise session, serum levels of anandamide were measured by Elisa. Results: Findings showed that the serum level of anandamide in the osteoarthritis patient group was significantly reduced compared to the healthy group that exercise and ozone therapy led to a significant increase in the serum anandamide level; but cell therapy could not have a significant effect on increasing serum levels of anandamide. The peak of this increase was seen in ozone+cell therapy and the interaction of all three interventions simultaneously. Conclusion: Although aerobic exercise and ozone therapy each independently have a positive effect on anandamide in people with osteoarthritis, but the interaction of all three interventions simultaneously with ozone+cell therapy is more effective.