Comparing the antimicrobial efficacy of different hand sanitizers: An In vitro Study

Background: The hands of health-care workers are the primary mode of transmission of the pathogens to patients, so hand hygiene is considered to be the most important and least expensive means of preventing nosocomial infections. Aim: The aim of this study is to assess and compare the antimicrobial efficacy of different hand sanitizers. Subjects and Methods: An in vitro study was conducted in the Department of Public Health Dentistry in the Dental College, Delhi NCR, to evaluate the antimicrobial efficacy of Green Apple (Himalaya), Lemon (Lifebuoy), Aloe vera (Tempo) hand sanitizers and compared them with the antimicrobial efficacy of sterillium hand sanitizers which is considered as the gold standard in hand sterilization. Twenty five participants were divided into five groups. The Gloves worn by the participants were swabbed after oral prophylaxis using different hand sanitizers. The inoculation of the sample was done and incubated for 48 h at 37°C, and colony-forming units were counted. The data obtained were compiled systematically using Microsoft Excel and were analyzed using the SPSS version 19.0. Data were statistically analyzed using descriptive statistics to assess the mean, standard deviation, standard error of the mean, and ANOVA and post hoc test for group-wise comparisons. A value of P < 0.05 was considered statistically significant. Results: Sterillium group showed the highest antimicrobial effectiveness followed by lemon group (71.4 ± 11.80), Green Apple group (112 ± 13.78), and A. vera group (156.4 ± 34.37), respectively, against all the organisms in the study. Conclusion: Sterillium possessed maximum antimicrobial effect against all the Gram-positive as well as Gram-negative bacteria as compared to lemon, green apple, and A. vera hand sanitizers.