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Antioxidant potential of Solanum nigrum aqueous leaves extract in modulating restraint stress-induced changes in rat’s liver

Authors :
Syed K Zaidi
Shakeel A Ansari
Shams Tabrez
Muhammad I Naseer
Moyad J Shahwan
Naheed Banu
Muhammad H Al-Qahtani
Source :
Journal of Pharmacy and Bioallied Sciences, Vol 11, Iss 1, Pp 60-68 (2019)
Publication Year :
2019
Publisher :
Wolters Kluwer Medknow Publications, 2019.

Abstract

Introduction: This study was carried out to evaluate the antioxidant potential of crude extract of Solanum nigrum leaves and its active constituents as treatment against restraint stress in rat’s liver. Methods: For this purpose, male albino Wistar rats were treated with crude extract of leaves and its alkaloid and flavonoid fractions both before and after 6 h of acute restraint stress. Prooxidant status of rat liver was assessed by determining the levels of thiobarbituric acid reactive substances, reduced glutathione, alkaline phosphatase, alanine transaminase, aspartate aminotransferase, and the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST). Results: Six hours of restraint stress generated oxidative stress in rat’s liver resulted in a significant rise in the level of the aforementioned liver enzymes. On the other hand, SOD, CAT, and GST enzymatic activities showed a significant decline in their level. The administration of crude leaves extract, both before and after stress exposure, significantly prevented the rise in the level of liver enzymes and reverted the activities of studied biochemical parameters toward their normal control values. However, the reversion was found to be more prominent in after-stress group. Conclusion: The aforementioned results highlight the significant antioxidant potential of S. nigrum extracts. On the basis of our study, we suggest the possible use of S. nigrum leaves extract as a nutritional supplement for combating oxidative stress induced damage.

Details

Language :
English
ISSN :
09757406
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Journal of Pharmacy and Bioallied Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.920190321d854065a755bdef6e33f4c2
Document Type :
article
Full Text :
https://doi.org/10.4103/JPBS.JPBS_58_18