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Circulating Mesenchymal Stromal Cells in Patients with Infantile Hemangioma: Evaluation of Their Functional Capacity and Gene Expression Profile

Authors :
Carlotta Abbà
Stefania Croce
Chiara Valsecchi
Elisa Lenta
Rita Campanelli
Alessia C. Codazzi
Valeria Brazzelli
Adriana Carolei
Paolo Catarsi
Gloria Acquafredda
Antonia Apicella
Laura Caliogna
Micaela Berni
Savina Mannarino
Maria A. Avanzini
Vittorio Rosti
Margherita Massa
Source :
Cells, Vol 13, Iss 3, p 254 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

We previously published that in patients with infantile hemangioma (IH) at the onset (T0) colony forming unit-fibroblasts (CFU-Fs) are present in in vitro cultures from PB. Herein, we characterize these CFU-Fs and investigate their potential role in IH pathogenesis, before and after propranolol therapy. The CFU-F phenotype (by flow cytometry), their differentiation capacity and ability to support angiogenesis (by in vitro cultures) and their gene expression (by RT-PCR) were evaluated. We found that CFU-Fs are actual circulating MSCs (cMSCs). In patients at T0, cMSCs had reduced adipogenic potential, supported the formation of tube-like structures in vitro and showed either inflammatory (IL1β and ESM1) or angiogenic (F3) gene expression higher than that of cMSCs from CTRLs. In patients receiving one-year propranolol therapy, the cMSC differentiation in adipocytes improved, while their support in in vitro tube-like formation was lost; no difference was found between patient and CTRL cMSC gene expressions. In conclusion, in patients with IH at T0 the cMSC reduced adipogenic potential, their support in angiogenic activity and the inflammatory/angiogenic gene expression may fuel the tumor growth. One-year propranolol therapy modifies this picture, suggesting cMSCs as one of the drug targets.

Details

Language :
English
ISSN :
20734409
Volume :
13
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Cells
Publication Type :
Academic Journal
Accession number :
edsdoj.916c9df12530413eaa41f0c489d6fa30
Document Type :
article
Full Text :
https://doi.org/10.3390/cells13030254