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Susceptibility Evaluation to Fire Blight and Genome-Wide Associations within a Collection of Asturian Apple Accessions

Authors :
Belén García-Fernández
Ramon Dolcet-Sanjuan
Diego Micheletti
María José Antón-Díaz
Cristina Solsona
Mercedes Fernández
Xavier Abad
Enrique Dapena
Source :
Plants, Vol 12, Iss 23, p 4068 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Fire blight, caused by Erwinia amylovora, is one of the most devastating apple diseases. The selection of cultivars of low susceptibility and the study of the genetic mechanisms of the disease play important roles in fire blight management. The susceptibility level to fire blight was evaluated in 102 accessions originating from Asturias, a cider-producing region located in the north of Spain with a wide apple germplasm. Evaluations took place under quarantine conditions using artificial inoculations of grafted plants. The results revealed wide variation in susceptibility responses and low-susceptible cultivars were identified. In addition, 91 cultivars were genotyped using the Affymetrix Axiom® Apple 480 K SNP array to conduct genome-wide association studies (GWAS). A statistically significant signal was detected on chromosome 10 using the multi-locus mixed model (MLMM). Two genes were identified as major putative candidate genes: a TIR-NBS-LRR class disease protein and a protein containing a development and cell death (DCD) domain. The outcomes of this study provide a promising source of information, particularly in the context of cider apples, and set a starting point for future genetic and breeding approaches.

Details

Language :
English
ISSN :
22237747
Volume :
12
Issue :
23
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.9086777c5c684d16b03aa73c6b4ab0a9
Document Type :
article
Full Text :
https://doi.org/10.3390/plants12234068