Back to Search Start Over

Comparative Analysis of QCM and Electrochemical Aptasensors for SARS-CoV-2 Detection

Authors :
Katarína Nemčeková
Jana Korčeková
Veronika Svitková
Denis Baraniak
Michaela Domšicová
Eva Melníková
Michaela Hornychová
Viktória Szebellaiová
Miroslav Gál
Alexandra Poturnayová
Source :
Biosensors, Vol 14, Iss 9, p 431 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

The rapid and accurate detection of SARS-CoV-2, particularly its spike receptor-binding domain (S-RBD), was crucial for managing the COVID-19 pandemic. This study presents the development and optimization of two types of aptasensors: quartz crystal microbalance (QCM) and electrochemical sensors, both employing thiol-modified DNA aptamers for S-RBD detection. The QCM aptasensor demonstrated exceptional sensitivity, achieved by optimizing aptamer concentration, buffer composition, and pre-treatment conditions, with a limit of detection (LOD) of 0.07 pg/mL and a linear range from 1 pg/mL to 0.1 µg/mL, and a significant frequency change was observed upon target binding. The electrochemical aptasensor, designed for rapid and efficient preparation, utilized a one-step modification process that reduced the preparation time to 2 h while maintaining high sensitivity and specificity. Electrochemical impedance spectroscopy (EIS) enabled the detection of S-RBD concentrations as low as 132 ng/mL. Both sensors exhibited high specificity, with negligible non-specific interactions observed in the presence of competing proteins. Additionally, the QCM aptasensor’s functionality and stability were verified in biological fluids, indicating its potential for real-world applications. This study highlights the comparative advantages of QCM and electrochemical aptasensors in terms of preparation time, sensitivity, and specificity, offering valuable insights for the development of rapid, sensitive, and specific diagnostic tools for the detection of SARS-CoV-2 and other viruses.

Details

Language :
English
ISSN :
20796374
Volume :
14
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.90617ef056794d6fa094e1b4305bbf88
Document Type :
article
Full Text :
https://doi.org/10.3390/bios14090431