Back to Search Start Over

Label-Free DNA Hybridization Detection Using a Highly Sensitive Fiber Microcavity Biosensor

Authors :
Yao Wu
Guiyu Wang
Xiujuan Yu
Yuanji Fan
Xuefeng Chen
Shengchun Liu
Source :
Sensors, Vol 24, Iss 1, p 278 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

A novel label-free optical fiber biosensor, based on a microcavity fiber Mach–Zehnder interferometer, was developed and practically demonstrated for DNA detection. The biosensor was fabricated using offset splicing standard communication single-mode fibers (SMFs). The light path of the sensor was influenced by the liquid sample in the offset open cavity. In the experiment, a high sensitivity of −17,905 nm/RIU was achieved in the refractive index (RI) measurement. On this basis, the probe DNA (pDNA) was immobilized onto the sensor’s surface using APTES, enabling real-time monitoring of captured complementary DNA (cDNA) samples. The experimental results demonstrate that the biosensor exhibited a high sensitivity of 0.32 nm/fM and a limit of detection of 48.9 aM. Meanwhile, the sensor has highly repeatable and specific performance. This work reports an easy-to-manufacture, ultrasensitive, and label-free DNA biosensor, which has significant potential applications in medical diagnostics, bioengineering, gene identification, environmental science, and other biological fields.

Details

Language :
English
ISSN :
24010278 and 14248220
Volume :
24
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Sensors
Publication Type :
Academic Journal
Accession number :
edsdoj.8fc215d2c45f445baa2ead8dbfa58ef1
Document Type :
article
Full Text :
https://doi.org/10.3390/s24010278