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New data analysis for BioSAXS at the ESRF
- Source :
- Journal of Synchrotron Radiation, Vol 29, Iss 5, Pp 1318-1328 (2022)
- Publication Year :
- 2022
- Publisher :
- International Union of Crystallography, 2022.
-
Abstract
- The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software has been rewritten to cope with the increased data flux and continues to provide beamline users with reduced and pre-processed data in real time. This article describes FreeSAS, an open-source collection of various small-angle scattering analysis algorithms needed to reduce and analyze BioSAXS data, and Dahu, the tool used to interface data analysis with beamline control. It further presents the data-processing pipelines for the different data acquisitions modes of the beamline, using either a sample changer for individual homogeneous samples or an inline size-exclusion chromatography setup.
- Subjects :
- biosaxs
online data analysis
solution scattering
proteins
biological small-angle x-ray scattering
automation
high brilliance
structural biology
high-throughput saxs
size-exclusion chromatography
online purification
Nuclear and particle physics. Atomic energy. Radioactivity
QC770-798
Crystallography
QD901-999
Subjects
Details
- Language :
- English
- ISSN :
- 16005775
- Volume :
- 29
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Synchrotron Radiation
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8e8ef0ebc8b469c94217d5c4bc683b3
- Document Type :
- article
- Full Text :
- https://doi.org/10.1107/S1600577522007238