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Burst sine wave electroporation (B-SWE) for expansive blood–brain barrier disruption and controlled non-thermal tissue ablation for neurological disease

Authors :
Sabrina N. Campelo
Zaid S. Salameh
Julio P. Arroyo
James L. May
Sara O. Altreuter
Jonathan Hinckley
Rafael V. Davalos
John H. Rossmeisl Jr.
Source :
APL Bioengineering, Vol 8, Iss 2, Pp 026117-026117-12 (2024)
Publication Year :
2024
Publisher :
AIP Publishing LLC, 2024.

Abstract

The blood–brain barrier (BBB) limits the efficacy of treatments for malignant brain tumors, necessitating innovative approaches to breach the barrier. This study introduces burst sine wave electroporation (B-SWE) as a strategic modality for controlled BBB disruption without extensive tissue ablation and compares it against conventional pulsed square wave electroporation-based technologies such as high-frequency irreversible electroporation (H-FIRE). Using an in vivo rodent model, B-SWE and H-FIRE effects on BBB disruption, tissue ablation, and neuromuscular contractions are compared. Equivalent waveforms were designed for direct comparison between the two pulsing schemes, revealing that B-SWE induces larger BBB disruption volumes while minimizing tissue ablation. While B-SWE exhibited heightened neuromuscular contractions when compared to equivalent H-FIRE waveforms, an additional low-dose B-SWE group demonstrated that a reduced potential can achieve similar levels of BBB disruption while minimizing neuromuscular contractions. Repair kinetics indicated faster closure post B-SWE-induced BBB disruption when compared to equivalent H-FIRE protocols, emphasizing B-SWE's transient and controllable nature. Additionally, finite element modeling illustrated the potential for extensive BBB disruption while reducing ablation using B-SWE. B-SWE presents a promising avenue for tailored BBB disruption with minimal tissue ablation, offering a nuanced approach for glioblastoma treatment and beyond.

Details

Language :
English
ISSN :
24732877
Volume :
8
Issue :
2
Database :
Directory of Open Access Journals
Journal :
APL Bioengineering
Publication Type :
Academic Journal
Accession number :
edsdoj.8dc2e8f3f7494a09b8d813d9ba8e45a0
Document Type :
article
Full Text :
https://doi.org/10.1063/5.0198382