Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

Authors :: Yaxiong Yang
Nan Liu
Yuanyuan He
Yuxia Liu
Lin Ge
Linzhi Zou
Sen Song
Wei Xiong
Xiaodong Liu
Source :: Nature Communications, Vol 9, Iss 1, Pp 1-18 (2018)
Publication Year :: 2018
Publisher :: Nature Portfolio, 2018.
Abstract: The popular genetically-encoded Ca2+ indicator, GCaMP, has several side-effects. Here the authors show that GCaMP containing CaM interferes with gating and signaling of L-type calcium channels, which disrupts Ca2+ dynamics and gene expression, and develop GCaMP-X to overcome these limitations.

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