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Rhodamine B triggers ovarian toxicity through oxidative stress, decreases in the number of follicles, 17B-estradiol level, and thickness of endometrium
- Source :
- Çukurova Üniversitesi Tıp Fakültesi Dergisi, Vol 39, Iss 3, Pp 451-457 (2014)
- Publication Year :
- 2014
- Publisher :
- Cukurova University, 2014.
-
Abstract
- Objective: The purpose of this study was to analyze the effects of exposure to rhodamine B on ovarian oxidative stress, ovarian follicles, hormone 17beta-estradiol and thickness of endometrium. Methods: A total of 28 female rats were divided into four groups consisting of control; groups treated with rhodamine B at doses of 4.5; 9, and 18 milligram/200 gram body weight. Rhodamine B was administered orally for 36 days with the probe. Analysis of MDA level was done spectrophotometrically. Analysis of the number of ovarian follicles and thickness of endometrium was done histopathologically by hematoxylin eosin staining. Analysis of 17-estradiol level was done by ELISA. Results: Rhodamine B administered in different doses in female rats can increase ovarian MDA levels significantly than the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce 17-estradiol level significantly compared to the control (P 0.05). The administration of rhodamine B could reduce thickness of endometrium significantly compared to the control (P 0.05). Conclusion: It was concluded that administration of rhodamine B triggered ovarian toxicity through oxidative stress, a decrease in the number of follicles, and decreased level of 17-estradiol which ultimately lowered the thickness of endometrium. [Cukurova Med J 2014; 39(3.000): 451-457]
- Subjects :
- dye
toxicity
ovarium
endometrium
oxidative stress
Medicine
Medicine (General)
R5-920
Subjects
Details
- Language :
- English, Turkish
- ISSN :
- 02505150
- Volume :
- 39
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Çukurova Üniversitesi Tıp Fakültesi Dergisi
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8c3502dff6914eeebdacf7cb56f1d412
- Document Type :
- article