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Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration
- Source :
- Neural Regeneration Research, Vol 14, Iss 9, Pp 1651-1656 (2019)
- Publication Year :
- 2019
- Publisher :
- Wolters Kluwer Medknow Publications, 2019.
-
Abstract
- MicroRNAs refer to a class of endogenous, short non-coding RNAs that mediate numerous biological functions. MicroRNAs regulate various physiological and pathological activities of peripheral nerves, including peripheral nerve repair and regeneration. Previously, using a rat sciatic nerve injury model, we identified many functionally annotated novel microRNAs, including miR-sc14. Here, we used real-time reverse transcription-polymerase chain reaction to examine miR-sc14 expression in rat sciatic nerve stumps. Our results show that miR-sc14 is noticeably altered following sciatic nerve injury, being up-regulated at 1 day and diminished at 7 days. EdU and transwell chamber assay results showed that miR-sc14 mimic promoted proliferation and migration of Schwann cells, while miR-sc14 inhibitor suppressed their proliferation and migration. Additionally, bioinformatic analysis examined potential target genes of miR-sc14, and found that fibroblast growth factor receptor 2 might be a potential target gene. Specifically, our results show changes of miR-sc14 expression in the sciatic nerve of rats at different time points after nerve injury. Appropriately, up-regulation of miR-sc14 promoted proliferation and migration of Schwann cells. Consequently, miR-sc14 may be an intervention target to promote repair of peripheral nerve injury. The study was approved by the Jiangsu Provincial Laboratory Animal Management Committee, China on March 4, 2015 (approval No. 20150304-004).
- Subjects :
- nerve regeneration
novel microRNAs
miR-sc14
peripheral nerve injury
cell proliferation
cell migration
Schwann cells
fibroblast growth factor receptor 2
biological functions
peripheral nerve regeneration
regulatory mechanisms
neural regeneration
Neurology. Diseases of the nervous system
RC346-429
Subjects
Details
- Language :
- English
- ISSN :
- 16735374
- Volume :
- 14
- Issue :
- 9
- Database :
- Directory of Open Access Journals
- Journal :
- Neural Regeneration Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8c223ff13c49441291ce0ff2f4f75675
- Document Type :
- article
- Full Text :
- https://doi.org/10.4103/1673-5374.255996