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Transcriptional Profiling of the Small Intestine and the Colon Reveals Modulation of Gut Infection with Citrobacter rodentium According to the Vitamin A Status

Authors :
Zhi Chai
Yafei Lyu
Qiuyan Chen
Cheng-Hsin Wei
Lindsay M. Snyder
Veronika Weaver
Aswathy Sebastian
István Albert
Qunhua Li
Margherita T. Cantorna
Catharine Ross
Source :
Nutrients, Vol 14, Iss 8, p 1563 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Vitamin A (VA) deficiency and diarrheal diseases are both serious public health issues worldwide. VA deficiency is associated with impaired intestinal barrier function and increased risk of mucosal infection-related mortality. The bioactive form of VA, retinoic acid, is a well-known regulator of mucosal integrity. Using Citrobacter rodentium-infected mice as a model for diarrheal diseases in humans, previous studies showed that VA-deficient (VAD) mice failed to clear C. rodentium as compared to their VA-sufficient (VAS) counterparts. However, the distinct intestinal gene responses that are dependent on the host’s VA status still need to be discovered. The mRNAs extracted from the small intestine (SI) and the colon were sequenced and analyzed on three levels: differential gene expression, enrichment, and co-expression. C. rodentium infection interacted differentially with VA status to alter colon gene expression. Novel functional categories downregulated by this pathogen were identified, highlighted by genes related to the metabolism of VA, vitamin D, and ion transport, including improper upregulation of Cl− secretion and disrupted HCO3− metabolism. Our results suggest that derangement of micronutrient metabolism and ion transport, together with the compromised immune responses in VAD hosts, may be responsible for the higher mortality to C. rodentium under conditions of inadequate VA.

Details

Language :
English
ISSN :
14081563 and 20726643
Volume :
14
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Nutrients
Publication Type :
Academic Journal
Accession number :
edsdoj.8c135fa3fb8846319a94f1bd21763f5b
Document Type :
article
Full Text :
https://doi.org/10.3390/nu14081563