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Direct supercritical angle localization microscopy for nanometer 3D superresolution

Authors :
Anindita Dasgupta
Joran Deschamps
Ulf Matti
Uwe Hübner
Jan Becker
Sebastian Strauss
Ralf Jungmann
Rainer Heintzmann
Jonas Ries
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Supercritical angle localisation microscopy (SALM) allows the z-positions of single fluorophores to be extracted from the intensity of supercritical angle fluorescence. Here the authors improve the z-resolution of SALM, and report nanometre isotropic localisation precision on DNA origami structures.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.8c05c23b63364577bddb90ef5a6f0f6d
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-21333-x