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Evaluation of an Enzyme-Linked Magnetic Electrochemical Assay for Hepatitis a Virus Detection in Drinking and Vegetable Processing Water

Authors :
Cristine D’Agostino
Rocco Cancelliere
Antonio Ceccarelli
Danila Moscone
Loredana Cozzi
Giuseppina La Rosa
Elisabetta Suffredini
Laura Micheli
Source :
Chemosensors, Vol 12, Iss 9, p 188 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Globally, waterborne viral infections significantly threaten public health. While current European Union regulations stipulate that drinking water must be devoid of harmful pathogens, they do not specifically address the presence of enteric viruses in water used for irrigation or food production. Traditional virus detection methods rely on molecular biology assays, requiring specialized personnel and laboratory facilities. Here, we describe an electrochemical sandwich enzyme-linked immunomagnetic assay (ELIME) for the detection of the hepatitis A virus (HAV) in water matrices. This method employed screen-printed electrodes as the sensing platform and utilized commercially available pre-activated magnetic beads to provide a robust foundation for the immunological reaction. The ELIME assay demonstrated exceptional analytical performance in only 185 min achieving a detection limit of 0.5 genomic copies per milliliter (g.c./mL) and exhibiting good reproducibility with a relative standard deviation (RSD) of 7% in HAV-spiked drinking and processing water samples. Compared with the real-time RT-qPCR method described in ISO 15216-1, the ELIME assay demonstrated higher sensitivity, although the overall linearity of the method was moderate. These analytical attributes highlight the potential of the ELIME assay as a rapid and viable alternative for HAV detection in water used for agriculture and food processing.

Details

Language :
English
ISSN :
22279040
Volume :
12
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Chemosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.8ba00bf51a6f4db09780e6145645e19b
Document Type :
article
Full Text :
https://doi.org/10.3390/chemosensors12090188