Back to Search
Start Over
Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains
Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains
- Source :
- Journal of Innovative Optical Health Sciences, Vol 16, Iss 06 (2023)
- Publication Year :
- 2023
- Publisher :
- World Scientific Publishing, 2023.
-
Abstract
- Multiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels. It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases. However, there has been no comparison with near-infrared (NIR) emission. In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo, we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828[Formula: see text]nm and 620[Formula: see text]nm, respectively. Both experimental and simulation results show that as the imaging depth increases, NIR emission decays less than orange-red fluorescent emission. These results show that it is preferable to shift the emission wavelength to NIR to enable more efficient signal collection deep in the brain.
Details
- Language :
- English
- ISSN :
- 17935458 and 17937205
- Volume :
- 16
- Issue :
- 06
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Innovative Optical Health Sciences
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8afa03895e8e44968cf125d36ea9970c
- Document Type :
- article
- Full Text :
- https://doi.org/10.1142/S1793545823400023