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Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains

Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains

Authors :
Ke Wang
Wanjian Zhang
Xiangquan Deng
Shen Tong
Hui Cheng
Mengyuan Qin
Lei Zheng
Kun Zhao
Ruizhan Zhai
Zhongqing Jia
Ping Qiu
Source :
Journal of Innovative Optical Health Sciences, Vol 16, Iss 06 (2023)
Publication Year :
2023
Publisher :
World Scientific Publishing, 2023.

Abstract

Multiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels. It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases. However, there has been no comparison with near-infrared (NIR) emission. In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo, we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828[Formula: see text]nm and 620[Formula: see text]nm, respectively. Both experimental and simulation results show that as the imaging depth increases, NIR emission decays less than orange-red fluorescent emission. These results show that it is preferable to shift the emission wavelength to NIR to enable more efficient signal collection deep in the brain.

Details

Language :
English
ISSN :
17935458 and 17937205
Volume :
16
Issue :
06
Database :
Directory of Open Access Journals
Journal :
Journal of Innovative Optical Health Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.8afa03895e8e44968cf125d36ea9970c
Document Type :
article
Full Text :
https://doi.org/10.1142/S1793545823400023