Assessing the synergistic effect of acidified carbon, inorganic fertilizer, and biofertilizer on fenugreek antioxidant levels, and quality traits

The use of inorganic fertilizers has become increasingly necessary due to the depletion of soil fertility. Inadequate nutrient levels in both plants and soil can be remedied with the application of fertilizers. However, to achieve optimal results, it is recommended to supplement inorganic fertilizers with organic alternatives such as biochar. Despite its benefits, the high pH of activated carbon presents a significant challenge to its use in alkaline soil. Recent studies have shown that acidified carbon can be used instead of alkaline activated carbon. Moreover, the use of biofertilizers is crucial for maintaining soil quality. The purpose of the current study was to investigate the effects of using biofertilizers, inorganic nitrogen fertilizer, and acidified carbon fertilizer in combination on fenugreek growth and chlorophyll concentrations. A pot experiment was conducted using calcium ammonium nitrate (CAN) and three levels of acidified carbon (BC) at 0, 0.75, and 1.50%, with and without the addition of biofertilizer (BF). The results revealed that 1.50CANBC + BF was the most effective supplement for improving soil characteristics such as pH, EC, and overall organic matter. The application of 0.75CANBC + BF was found to be a successful treatment for promoting fenugreek growth and enhancing nitrogen absorption. To achieve better fenugreek quality by reducing electrolyte leakage and MDA, and increasing chlorophyll levels, gardeners are advised to use 1.50CANBC + BF. However, to establish 1.50CANBC + BF as the optimal method for fenugreek growth in poor fertility and high pH soils, further experimental studies in various agro-climatic conditions are necessary.