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An atlas of brain-bone sympathetic neural circuits in mice

Authors :
Vitaly Ryu
Anisa Azatovna Gumerova
Ronit Witztum
Funda Korkmaz
Liam Cullen
Hasni Kannangara
Ofer Moldavski
Orly Barak
Daria Lizneva
Ki A Goosens
Sarah Stanley
Se-Min Kim
Tony Yuen
Mone Zaidi
Source :
eLife, Vol 13 (2024)
Publication Year :
2024
Publisher :
eLife Sciences Publications Ltd, 2024.

Abstract

There is clear evidence that the sympathetic nervous system (SNS) mediates bone metabolism. Histological studies show abundant SNS innervation of the periosteum and bone marrow–these nerves consist of noradrenergic fibers that immunostain for tyrosine hydroxylase, dopamine beta-hydroxylase, or neuropeptide Y. Nonetheless, the brain sites that send efferent SNS outflow to the bone have not yet been characterized. Using pseudorabies (PRV) viral transneuronal tracing, we report, for the first time, the identification of central SNS outflow sites that innervate bone. We find that the central SNS outflow to bone originates from 87 brain nuclei, sub-nuclei, and regions of six brain divisions, namely the midbrain and pons, hypothalamus, hindbrain medulla, forebrain, cerebral cortex, and thalamus. We also find that certain sites, such as the raphe magnus (RMg) of the medulla and periaqueductal gray (PAG) of the midbrain, display greater degrees of PRV152 infection, suggesting that there is considerable site-specific variation in the levels of central SNS outflow to the bone. This comprehensive compendium illustrating the central coding and control of SNS efferent signals to bone should allow for a greater understanding of the neural regulation of bone metabolism, and importantly and of clinical relevance, mechanisms for central bone pain.

Details

Language :
English
ISSN :
2050084X
Volume :
13
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.8a653d789f845e8876a995a110d6788
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.95727