Back to Search Start Over

Differential Neutralization of Unfractionated Heparin and Enoxaparin by Andexanet Alfa

Authors :
Joseph Lewis BA
Omer Iqbal MD
Walter Jeske PhD
Debra Hoppensteadt PhD
Fakiha Siddiqui BDS
Jawed Fareed PhD
Source :
Clinical and Applied Thrombosis/Hemostasis, Vol 28 (2022)
Publication Year :
2022
Publisher :
SAGE Publishing, 2022.

Abstract

Introduction Andexanet alfa (andexanet) is an approved antidote used to reverse the bleeding effects of Direct Oral Anticoagulant (Direct-Xa agents) agents because it reverses anti-Xa activity. Unfractionated heparin (UFH) and low molecular weight heparins (LMWHs) exhibit anti-Xa activity. The purpose is to investigate the neutralization of UFH and LMWH by andexanet in activated clotting time (ACT), thrombelastography (TEG), and anti-Xa due to the protamine sulfate shortage. Methods UFH and LMWH were studied with andexanet, PS, or saline as potential reversal agents/controls at varying concentrations in ACT, TEG, and anti-Xa and compared to each other. Results Andexanet partially neutralized both drugs several TEG parameters at high andexanet concentrations, but it was not as effective as protamine sulfate in any of the assays used. Most TEG parameters were correlated with andexanet concentration. In ACT, significant neutralization was demonstrated at many andexanet concentrations for UFH, but not LMWH. UFH was completely neutralized by PS in ACT, while LMWH was partially neutralized by PS in ACT. Andexanet alfa was a less effective neutralization agent than the protamine sulfate as it only partially neutralized UFH in ACT and was ineffective at neutralizing LMWH when tested at the same concentration as PS (10 ug/mL). Conclusion Andexanet partially neutralized UFH and LMWH with variability between assays, necessitating investigation into assay-dependent differences.

Details

Language :
English
ISSN :
19382723 and 10760296
Volume :
28
Database :
Directory of Open Access Journals
Journal :
Clinical and Applied Thrombosis/Hemostasis
Publication Type :
Academic Journal
Accession number :
edsdoj.898573021cb94fc8992380467d649964
Document Type :
article
Full Text :
https://doi.org/10.1177/10760296221099934