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Expression of Fascin and SALL4 in odontogenic cysts and tumors: an immunohistochemical appraisal. [version 5; peer review: 1 approved, 2 approved with reservations, 2 not approved]

Authors :
Harishanker Alampally
Vasudev Guddattu
Spoorti Kulkarni
Gabriel Rodrigues
Sunitha Carnelio
Source :
F1000Research, Vol 11 (2024)
Publication Year :
2024
Publisher :
F1000 Research Ltd, 2024.

Abstract

Background Various stemness markers (SOX2, OCT4, and NANOG) have been studied in odontogenic cysts and tumors. However, studies on SALL4 having similar properties of stemness has not been documented. Additionally, insight into fascin as a migratory molecule is less explored. In this study, the expression of SALL4 and fascin were evaluated in ameloblastoma, adenomatoid odontogenic tumor (AOT), odontogenic keratocyst (OKC), dentigerous cyst (DC), radicular cyst (RC), and calcifying odontogenic cyst (COC). Methods Semi-quantitative analysis of fascin and SALL4 immuno-positive cells was done in a total of 40 cases of ameloblastoma (11 plexiform, 12 follicular, 12 unicystic, and 5 desmoplastic) variants, 6 cases of AOT, 15 each of OKC, DC, RC and 5 of COC. Chi-square test was applied to evaluate the association between SALL4 and fascin expression in odontogenic cysts and tumors. Results Fascin immunopositivity was observed in peripheral ameloblast-like cells, and the expression was weak or absent in stellate reticulum-like cells. A moderate to weak immune-reactivity to SALL4 was observed in the cytoplasm of ameloblastoma, epithelial cells of dentigerous and radicular cysts, having a marked inflammatory infiltrate, which was an interesting observation. COC and AOT had negative to weak expressions. No recurrence has been reported. Conclusions Expression of fascin in ameloblastomas elucidate their role in motility and localized invasion. Its expression in less aggressive lesions like DC, COC, AOT will incite to explore the other functional properties of fascin. SALL4 expression in the cytoplasm of odontogenic cysts and tumors may represent inactive or mutant forms which requires further validation.

Details

Language :
English
ISSN :
20461402
Volume :
11
Database :
Directory of Open Access Journals
Journal :
F1000Research
Publication Type :
Academic Journal
Accession number :
edsdoj.88dd81239f6e43c18b8c3f3fde5caf76
Document Type :
article
Full Text :
https://doi.org/10.12688/f1000research.126091.5