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Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris

Authors :
Qi Liu
Ziyu He
Menghao Cai
Source :
Synthetic and Systems Biotechnology, Vol 10, Iss 1, Pp 133-139 (2025)
Publication Year :
2025
Publisher :
KeAi Communications Co., Ltd., 2025.

Abstract

Methanol is a promising substrate for sustainable biomanufacturing, and Pichia pastoris has become a commonly used yeast for methanol utilization due to its powerful methanol metabolic pathways and methanol inducible promoter. Previous reconstruction of gene circuits highly improved transcriptional activity, but excessive expression of chimeric transactivator damaged cell growth on methanol. Here we employed transcriptome analysis to investigate the effects of chimeric transactivator overexpression on cellular metabolism and regulatory networks. The results showed that strong expression of chimeric transactivator unexpectedly downregulated methanol metabolism, especially the alcohol oxidase 1 (AOX1), but without remarkable changes in expression of transcriptional factors. Meanwhile, the synthesis of peroxisomes also varied with chimeric transactivator expression. In addition, the enrichment analysis of differentially expressed genes revealed their impact on cellular metabolism. The gene expression patterns caused by different expression levels of chimeric transactivators have also been clarified. This work provides useful information to understand the transcriptional regulation of the AOX1 promoter and methanol signaling. It revealed the importance of balancing transcription factor expression for the host improvement.

Details

Language :
English
ISSN :
2405805X
Volume :
10
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Synthetic and Systems Biotechnology
Publication Type :
Academic Journal
Accession number :
edsdoj.886668d77c004876b3a9bf961f5fa3af
Document Type :
article
Full Text :
https://doi.org/10.1016/j.synbio.2024.09.008